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41.
42.
Koji Suzuki Akira Hinuma Hiroaki Saito Hiroshi Kiyosawa Hongbin Liu Toshiro Saino Atsushi Tsuda 《Progress in Oceanography》2005,64(2-4):167
To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l−1 in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l−1 in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l−1 at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment. 相似文献
43.
南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究 总被引:14,自引:0,他引:14
从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0~20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6~9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用. 相似文献
44.
Mussel Periostracum from Deep-Sea Redox Communities as a Microbial Habitat: 3. Secondary Inhabitants
Abstract. Vacated microborings in periostracum of live mussels of the Florida Escarpment redox community (depth of 3266 m) become a habitat for a prolific secondary microbiota consisting of a variety of prokaryotic and a few eukaryotic organisms. Periostracum surface is also colonized by diverse microorganisms, dominated by presumed chemolithotrophic bacteria with stacks of intracellular lamellae. Unlike sheltered microflora within borings, the surface community is heavily grazed upon by numerous archaeogastropods and ciliates. 相似文献
45.
水生动物对植物蛋白源利用的研究进展 总被引:17,自引:1,他引:17
综述了水生动物对植物蛋白源的利用.饲料中植物蛋白水平将显著影响鱼类的生长和生理生化状态.水生动物对植物蛋白源的利用与适口性、消化率和必需氨基酸平衡相关.同进实验动物的种类、食性和发育阶段均会对植物蛋白利用产生一定影响.水生动物饲料中植物蛋白源的适宜添加量主要受实验动物种类和饲料中蛋白质水平的影响.因此,通过添加限制性必需氨基酸和减少搞营养因子可以提高水生动物对植物蛋白源的利用. 相似文献
46.
47.
Symbiotic Relations of Sediment-Agglutinating Nematodes and Bacteria in Detrital Habitats: The Enzyme-Sharing Concept 总被引:2,自引:0,他引:2
Abstract. A new concept (‘enzyme sharing’) concerning the interaction of marine nematodes and microbes in the degradation of sedimentary detritus is presented. Elements of this concept are (1) the notorious tendency of many aquatic nematodes to agglutinate detrital particles by mucus secretions, (2) new observations on the stimulation of microbial growth by nematodes in agar plates, and (3) literature data on limited endogenous proteolytic capacities of aquatic nematodes. Observations on nematode‐microbe associations in agar plates prompted the conceptual synthesis. In agar medium without the addition of any nutrients a spectacular growth of bacteria was visible on the sinusoidal crawling trails of nematodes only 2 – 3 days after introduction of the worms (species of Adoncholaimus, Anoplostoma and Sabatieria). Juveniles of Anoplostoma that hatched in the agar cultures left their minute trails in the medium and these were rapidly occupied by bacteria. The nematodes repeatedly visited their bacterial trails, which persisted as a peculiar biotic structure for more than one year and survived the nematodes.In sterile agar preparations containing the fluorogenic methylumbelliferyl‐β‐glucoside in the presence of the nematode Adoncholaimus, an enhanced fluorescence of the medium was visible, indicating β‐glucosidase activity. We therefore assume that oncholaimid nematodes discharge enzymes that alone, or in concert with microbial activities, contribute to the hydrolytic cleavage of refractory polysaccharides containing β‐glucosidic bonds such as agar components and cellulose. The sugars thus produced may then be taken up by the nematodes and concomitantly support the conspicuous growth of microbes. Since we did not observe any feeding of the nematodes on the associated microbes in agar plates, we question the nutritive potential of intact microbial cells for a number of nematodes abounding in detrital habitats, and call attention to the significance of ambient dissolved or adsorbed organic monomeric nutrients.Consequently, we perceive the puzzling perpetual accretion of detrital organic particles to sediment agglutinations by nematodes as an adaptation for operating an ‘enzymatic reactor’ for the production of dissolved nutrients. We hypothesise a relationship of mutual commensalism of nematodes and heterotrophic microbes in detrital habitats and propose the term ‘enzyme sharing’ for this relationship. Both parties invest in a common enzyme pool that decomposes organic detritus for their nutrition. We present here evidence that nematodes contribute β‐glucosidase, which is involved in the cellulase system. Data from the literature suggest that microbial enzymatic processing of detrital proteins yields amino acids available to nematodes, which apparently have no efficient proteolytic enzyme system in their intestines. 相似文献
48.
阐述把维生素B12生产菌添加到培养水中培养褶皱臂尾轮虫BrachionusPlicatilis的买验。共18株细菌分离于轮虫培养池,其中,有一株产维生素B12的假单胞杆菌TP4对轮虫的生长繁殖有明显的促进作用。把TP4菌株培养后,加入到2L的烧杯和500L的水槽中培养泰国S型轮虫时.在9d(天)和6d(天)中,轮虫密度从124~139和242~288个体/ml增殖到4,417~5,540和1,017~1,254个体/ml,分别比对照组增加了4~6及2~3倍。 相似文献
49.
F. D. Por 《Marine Ecology》1980,1(2):121-131
Abstract. A classification of hypersaline waters is proposed on the basis of the food chain structure of their biota. Four steps of increasing salinity and respective shortening of the food-chains are recognized, respectively named: alpha-, beta-, gamma- and delta-hypersaline waters.
Some general aspects, dealing with cosmopolitism of the biota, production, competition and benthic ecology in hypersaline waters are discussed.
The relevance of ecological studies in hypersaline waters to theoretical ecology and to the new technological uses of saline waters is briefly mentioned. 相似文献
Some general aspects, dealing with cosmopolitism of the biota, production, competition and benthic ecology in hypersaline waters are discussed.
The relevance of ecological studies in hypersaline waters to theoretical ecology and to the new technological uses of saline waters is briefly mentioned. 相似文献
50.
Young-Ok Kim Eun Jin Yang Jung-Hoon Kang Kyoungsoon Shin Man Chang Cheol Soo Myung 《Ocean Science Journal》2007,42(1):9-17
The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and
heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake
Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner
area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L1) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher
densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while
microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed
into more than 106 cells L1 at the surface layer of the inner area, while its abundance was much lower (103-104 cells L1) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p <
0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing
of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates. 相似文献